Journal:

Article Title: Th1 Cytokines Facilitate CD8-T-Cell-Mediated Early Resistance to Infection with Mycobacterium tuberculosis in Old Mice

doi: 10.1128/IAI.01475-05

Figure Lengend Snippet: Th1 cytokine production in the lungs of old mice during infection with M. tuberculosis. Young and old mice were aerogenically infected with a low dose of M. tuberculosis Erdman. At specific times after infection the right middle lung lobe was removed, homogenized in Ultraspec, and frozen at −80°C. RNA was isolated and reverse transcribed, and cDNA for IL-12p40 (A) and IFN-γ (B) was amplified by real-time PCR. The data are means ± standard errors for the relative quantity of message from five mice at each time and are representative of three independent experiments. 18S was used as an endogenous normalizer, and naïve young mice were used as the calibrator. At the early times after infection (0 to 12 days) the total numbers of lung cells isolated from young and old mice were statistically indistinguishable (data not shown). (C and D) On day 12 postinfection aliquots of lung homogenate were analyzed to determine the levels of IL-12p40 (C) and IL-18 (D) protein by ELISA. The data are means ± standard errors for five mice per group and are representative of two independent experiments. An asterisk indicates that the P value is <0.05, as determined by Student's t test.

Article Snippet: M. tuberculosis Erdman (= ATCC 35801) was obtained from American Type Culture Collection (Manassas, VA) and grown in Proskauer-Beck liquid medium containing 0.05% Tween 80 to the mid-log phase.

Techniques: Infection, Isolation, Reverse Transcription, Amplification, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

Journal:

Article Title: Th1 Cytokines Facilitate CD8-T-Cell-Mediated Early Resistance to Infection with Mycobacterium tuberculosis in Old Mice

doi: 10.1128/IAI.01475-05

Figure Lengend Snippet: Early resistance of old mice to M. tuberculosis infection. Young and old mice were infected with a low dose of M. tuberculosis Erdman by aerosol, and bacterial burdens in the lungs were determined (A). The data are means ± standard errors for five mice per group and are representative of four independent experiments. BrdU was administered intraperitoneally 14 h before lung cells were harvested. (B and C) Single-cell suspensions from lungs were stained with fluorescently conjugated antibodies against CD3ɛ, CD8, and BrdU. (C) Representative dot plots for lung cells from young and old mice on day 15 postinfection. (B) Percentages were determined and used to calculate absolute numbers. The data are representative of two independent experiments. One asterisk indicates that the P value is <0.05 and three asterisks indicate that the P value is <0.0005, as determined by Student's t test.

Article Snippet: M. tuberculosis Erdman (= ATCC 35801) was obtained from American Type Culture Collection (Manassas, VA) and grown in Proskauer-Beck liquid medium containing 0.05% Tween 80 to the mid-log phase.

Techniques: Infection, Aerosol, Staining

Journal: PLoS ONE

Article Title: Location of Intra- and Extracellular M. tuberculosis Populations in Lungs of Mice and Guinea Pigs during Disease Progression and after Drug Treatment

doi: 10.1371/journal.pone.0017550

Figure Lengend Snippet: Mice were infected with M. tuberculosis strain Erdman and treated with drugs starting 18 to 21 days after aerosol infection; with isoniazid (INH), rifampin (RIF), ethambutol (EMB), gatifloxacin (Gati) or moxifloxacin (Moxi). Sacrifice times were at 2, 5 and 7 days of drug treatment. Data points represent mean log 10 viable bacilli +/− standard error present in whole lung homogenates.

Article Snippet: The virulent M. tuberculosis Erdman strain (TMC 107; ATCC 35801) has been used as a standard for drug evaluations in mice in our laboratory, and the strain was propagated as previously described , .

Techniques: Infection, Aerosol

Journal:

Article Title: In Vitro Antimycobacterial Activity of 5-Chloropyrazinamide

doi:

Figure Lengend Snippet: MICs of pyrazinamide analogs for various mycobacteria

Article Snippet: Strains of M. tuberculosis (ATCC 27294, ATCC 35801, and ATCC 35828), M. bovis (ATCC 35720 and ATCC 27289), Mycobacterium smegmatis (ATCC 19420), and Mycobacterium fortuitum (ATCC 49403) were obtained from the American Type Culture Collection, Rockville, Md.

Techniques: